Hotspot

petB

Genome · 71979-73454 · 1476 bp

Back to Asplenium

Pi 0.0615

MarkerSeek score 84.50

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: Genome
length_bp: 1476
variable_sites: 283
pi: 0.0615
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CAATCAATCGAGCAGGCTCT	GTCACACACTCCCGTAATCC	50.0	55.0	58.0	58.0	1973-2051 bp	1.0	91.6
petB_p2	TCAATCAATCGAGCAGGCTC	GTCACACACTCCCGTAATCC	50.0	55.0	58.1	58.0	1974-2052 bp	1.0	91.5
petB_p3	TACAAAGCGTAACCGGTGAG	GTCACACACTCCCGTAATCC	50.0	55.0	57.9	58.0	2121-2193 bp	1.0	91.4
petB_p4	CAATCAATCGAGCAGGCTCT	GTGGAACAAAGCTTCCTGGA	50.0	50.0	58.0	58.0	2429-2485 bp	0.25	56.0
petB_p5	TCAATCAATCGAGCAGGCTC	GTGGAACAAAGCTTCCTGGA	50.0	50.0	58.1	58.0	2430-2486 bp	0.25	56.0