Hotspot

rps12-rrn16

Genome · 139200-140412 · 1213 bp

Back to Asplenium

Pi 0.0512

MarkerSeek score 86.50

Primer available yes

Label rps12-rrn16

Feature Metadata

label_name: rps12-rrn16
feature_type: igs
region: Genome
length_bp: 1213
variable_sites: 223
pi: 0.0512
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rrn16_p1	GAACTCTCGCGACTTTACGT	TCCGACTTGCATGTGTTAGG	50.0	50.0	58.0	57.9	1148-1346 bp	0.833333	84.3
rps12-rrn16_p2	CTCTCGCGACTTTACGTAGG	TCCGACTTGCATGTGTTAGG	55.0	50.0	57.9	57.9	1145-1343 bp	0.833333	83.9
rps12-rrn16_p3	AGCCGTTACCTCAAACTTGG	TCCGACTTGCATGTGTTAGG	50.0	50.0	58.1	57.9	1177-1375 bp	0.833333	83.7
rps12-rrn16_p4	GCCGTTACCTCAAACTTGGA	TCCGACTTGCATGTGTTAGG	50.0	50.0	58.1	57.9	1176-1374 bp	0.833333	83.7
rps12-rrn16_p5	GAGGTTAGTCGAACTCTCGC	TCCGACTTGCATGTGTTAGG	55.0	50.0	57.8	57.9	1158-1356 bp	0.833333	83.2