Hotspot

rpl32-trnP-GGG

Genome · 109141-109752 · 612 bp

Back to Asplenium

Pi 0.176

MarkerSeek score 80.30

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: Genome
length_bp: 612
variable_sites: 282
pi: 0.176
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	AGAAGATGGCAATACAGCGG	TATCACGGAATATGGCGCAG	50.0	50.0	58.0	57.9	913-1133 bp	0.666667	77.7
rpl32-trnP-GGG_p2	GAAGATGGCAATACAGCGGA	TATCACGGAATATGGCGCAG	50.0	50.0	58.0	57.9	912-1132 bp	0.5	71.0
rpl32-trnP-GGG_p3	AGAAGATGGCAATACAGCGG	GAACGGGTGCTGGCATATAA	50.0	50.0	58.0	58.0	1184 bp	0.083333	42.7
rpl32-trnP-GGG_p4	GAAGATGGCAATACAGCGGA	GAACGGGTGCTGGCATATAA	50.0	50.0	58.0	58.0	1183 bp	0.083333	42.7
rpl32-trnP-GGG_p5	ATCGGTGGTAAACAACTCCG	GAACGGGTGCTGGCATATAA	50.0	50.0	57.9	58.0	1389 bp	0.083333	42.3