Hotspot

trnT-GGU-trnfM-CAU

Genome · 34976-35448 · 473 bp

Back to Asplenium

Pi 0.148

MarkerSeek score 77.60

Primer available yes

Label trnT-GGU-trnfM-CAU

Feature Metadata

label_name: trnT-GGU-trnfM-CAU
feature_type: igs
region: Genome
length_bp: 473
variable_sites: 198
pi: 0.148
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnfM-CAU_p1	GAACCGATGACTTACGCCTT	CCAGACATGTACTTCGCGAA	50.0	50.0	58.0	58.0	767-807 bp	0.666667	78.5
trnT-GGU-trnfM-CAU_p2	GAACCGATGACTTACGCCTT	TTTACCGCGTAGTAGTGCAC	50.0	50.0	58.0	58.0	856-896 bp	0.666667	78.5
trnT-GGU-trnfM-CAU_p3	TAAGCATCCGCAGTGTAACC	CCAGACATGTACTTCGCGAA	50.0	50.0	58.0	58.0	1271 bp	0.083333	43.1
trnT-GGU-trnfM-CAU_p4	TAAGCATCCGCAGTGTAACC	TTTACCGCGTAGTAGTGCAC	50.0	50.0	58.0	58.0	1360 bp	0.083333	43.0
trnT-GGU-trnfM-CAU_p5	GAACCGATGACTTACGCCTT	ACCCTTCCCACTAGCTCTTT	50.0	50.0	58.0	58.0	1201 bp	0.083333	42.9