Hotspot

psbD-trnT-GGU

Genome · 33819-34903 · 1085 bp

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Pi 0.101

MarkerSeek score 81.30

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1085
variable_sites: 330
pi: 0.101
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TAGTAAGCCAGACCAACCCA	AAGGCATTAACTAAGCGGGG	50.0	50.0	58.0	57.9	1805-2111 bp	0.916667	88.0
psbD-trnT-GGU_p2	GAGATCTCCTTGTGCTTCGG	AAGGCATTAACTAAGCGGGG	55.0	50.0	58.1	57.9	2000-2306 bp	0.916667	87.7
psbD-trnT-GGU_p3	CTGTGAACCAACCTCCCAAA	AAGGCATTAACTAAGCGGGG	50.0	50.0	57.9	57.9	1848-2129 bp	0.416667	67.7
psbD-trnT-GGU_p4	TGTGAACCAACCTCCCAAAG	AAGGCATTAACTAAGCGGGG	50.0	50.0	57.9	57.9	1847-2128 bp	0.416667	67.7
psbD-trnT-GGU_p5	TAGTAAGCCAGACCAACCCA	GCTTAGTTGCCTTCACCGTA	50.0	50.0	58.0	57.9	1303-1339 bp	0.166667	53.2