Hotspot

trnC-GCA-trnG-GCC

Genome · 29784-30204 · 421 bp

Back to Asplenium

Pi 0.122

MarkerSeek score 78.30

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: Genome
length_bp: 421
variable_sites: 155
pi: 0.122
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	GATTTGCAGTCCTCCGTCTT	GTTCGATTCCCGCTATCCG	50.0	57.9	57.9	58.2	415-495 bp	1.0	81.7
trnC-GCA-trnG-GCC_p2	GATTTGCAGTCCTCCGTCTT	GGGTTCGATTCCCGCTATC	50.0	57.9	57.9	57.8	417-497 bp	1.0	81.5
trnC-GCA-trnG-GCC_p3	GATTTGCAGTCCTCCGTCTT	GGTTCGATTCCCGCTATCC	50.0	57.9	57.9	57.8	416-496 bp	1.0	81.5
trnC-GCA-trnG-GCC_p4	TCCGTCTTACCGCTTGACTA	GTTCGATTCCCGCTATCCG	50.0	57.9	58.2	58.2	403-483 bp	1.0	81.1
trnC-GCA-trnG-GCC_p5	TCCGTCTTACCGCTTGACTA	GGTTCGATTCCCGCTATCC	50.0	57.9	58.2	57.8	404-484 bp	1.0	80.9