Hotspot

psbM-petN

Genome · 27914-29122 · 1209 bp

Back to Asplenium

Pi 0.11

MarkerSeek score 80.50

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 1209
variable_sites: 401
pi: 0.11
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TCTCTACATTCAAACGGCCG	AAGACGGAGGACTGCAAATC	50.0	50.0	58.0	57.9	1768-1925 bp	1.0	91.2
psbM-petN_p2	TCTCTACATTCAAACGGCCG	TAGTCAAGCGGTAAGACGGA	50.0	50.0	58.0	58.2	1780-1937 bp	1.0	90.6
psbM-petN_p3	TCTCTACATTCAAACGGCCG	TGTCTCAATTCGTCACGCAT	50.0	45.0	58.0	57.9	1655-1695 bp	0.25	58.6
psbM-petN_p4	TCTCTACATTCAAACGGCCG	TGTGAAAGTGTGAAAGGCGA	50.0	45.0	58.0	58.0	1919-1959 bp	0.25	58.4
psbM-petN_p5	TCTAAACGATGGGGGCTTTG	TGTGAAAGTGTGAAAGGCGA	50.0	45.0	57.9	58.0	2440-2473 bp	0.25	57.2