Hotspot

trnL-CAA-ycf2

IRa · 150416-151377 · 962 bp

Back to Aspidopterys

Pi 0.0156

MarkerSeek score 48.20

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 962
variable_sites: 30
pi: 0.0156
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	GTTCGAGTCCTCTTCAAGGC	GGATGAAATGAAAATTGGATTCATGT	55.0	30.8	58.3	57.3	1003-1011 bp	1.0	43.0
trnL-CAA-ycf2_p2	CTAAAGAGCGTGGAGGTTCG	GGATGAAATGAAAATTGGATTCATGT	55.0	30.8	58.4	57.3	1018-1026 bp	1.0	43.0
trnL-CAA-ycf2_p3	GCTAAAGAGCGTGGAGGTTC	GGATGAAATGAAAATTGGATTCATGT	55.0	30.8	58.6	57.3	1019-1027 bp	1.0	43.0
trnL-CAA-ycf2_p4	TTCGAGTCCTCTTCAAGGCA	GGATGAAATGAAAATTGGATTCATGT	50.0	30.8	58.7	57.3	1002-1010 bp	1.0	43.0
trnL-CAA-ycf2_p5	GTTCGAGTCCTCTTCAAGGC	GGATGAAATGAAAATTGGATTCA	55.0	30.4	58.3	53.9	1003-1011 bp	1.0	43.0