Hotspot

trnL-UAG-ndhF

SSC · 129857-131681 · 1825 bp

Back to Aspidopterys

Pi 0.013

MarkerSeek score 63.50

Primer available yes

Label trnL-UAG-ndhF

Feature Metadata

label_name: trnL-UAG-ndhF
feature_type: igs
region: SSC
length_bp: 1825
variable_sites: 44
pi: 0.013
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ndhF_p1	AGCGTGTCTACCGATTTCAC	AGAAGTCCCGCCCCTATTAA	50.0	50.0	58.0	57.8	1749-1936 bp	1.0	84.3
trnL-UAG-ndhF_p2	CAGCGTGTCTACCGATTTCA	AGAAGTCCCGCCCCTATTAA	50.0	50.0	58.0	57.8	1750-1937 bp	1.0	84.3
trnL-UAG-ndhF_p3	AGCGTGTCTACCGATTTCAC	AAAAAGTAGAAGTCCCGCCC	50.0	50.0	58.0	57.8	1756-1943 bp	1.0	84.2
trnL-UAG-ndhF_p4	CAGCGTGTCTACCGATTTCA	AAAAAGTAGAAGTCCCGCCC	50.0	50.0	58.0	57.8	1757-1944 bp	1.0	84.2
trnL-UAG-ndhF_p5	TCGAACCGAGATGCTTTAGC	AGAAGTCCCGCCCCTATTAA	50.0	50.0	58.1	57.8	1785-1972 bp	1.0	83.7