Hotspot

petD-rpoA

LSC · 82709-82846 · 138 bp

Back to Aspidopterys

Pi 0.0399

MarkerSeek score 63.00

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 138
variable_sites: 11
pi: 0.0399
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GTAGTGGCCCTTTGGTTAGG	GCACTTTGCAATTGATTTACCA	55.0	36.4	57.9	56.4	292 bp	1.0	53.2
petD-rpoA_p2	AGTGGCCCTTTGGTTAGGTA	GCACTTTGCAATTGATTTACCA	50.0	36.4	58.2	56.4	290 bp	1.0	52.2
petD-rpoA_p3	TAGTGGCCCTTTGGTTAGGT	GCACTTTGCAATTGATTTACCA	50.0	36.4	58.2	56.4	291 bp	1.0	52.2
petD-rpoA_p4	CGTAGTGGCCCTTTGGTTAG	GCACTTTGCAATTGATTTACCA	55.0	36.4	58.3	56.4	293 bp	1.0	52.1
petD-rpoA_p5	GTAGTGGCCCTTTGGTTAGG	GCACTTTGCAATTGATTTACCAA	55.0	34.8	57.9	57.1	292 bp	1.0	50.7