Hotspot

trnW-CCA-trnP-UGG

LSC · 71005-71187 · 183 bp

Back to Aspidopterys

Pi 0.00638
MarkerSeek score 44.50
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
183
variable_sites
2
pi
0.00638
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 247-252 bp 1.0 81.1
trnW-CCA-trnP-UGG_p2 GTTTTGGAGACCCACGTTCT CACGGGTTCAAATCCTGTCA 50.0 50.0 58.0 58.1 245-250 bp 1.0 81.1
trnW-CCA-trnP-UGG_p3 GTTTTGGAGACCCACGTTCT TGTCACGGGTTCAAATCCTG 50.0 50.0 58.0 58.1 248-253 bp 1.0 81.1
trnW-CCA-trnP-UGG_p4 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 258-263 bp 1.0 81.0
trnW-CCA-trnP-UGG_p5 GTTTTGGAGACCCACGTTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 259-264 bp 1.0 81.0