Hotspot

petG-trnW-CCA

LSC · 70817-70930 · 114 bp

Back to Aspidopterys

Pi 0.0819

MarkerSeek score 85.50

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 114
variable_sites: 14
pi: 0.0819
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GTGGTGATCAGTTGGACCTT	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.4	58.0	182-191 bp	1.0	84.9
petG-trnW-CCA_p2	GACGTGGTGATCAGTTGGAC	AGAACGTGGGTCTCCAAAAC	55.0	50.0	58.6	58.0	185-194 bp	1.0	84.7
petG-trnW-CCA_p3	ACTTTGGCCGGATTATTCGT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	57.6	58.0	225-234 bp	1.0	84.3
petG-trnW-CCA_p4	AGACGTGGTGATCAGTTGGA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.7	58.0	186-195 bp	1.0	84.0
petG-trnW-CCA_p5	ACTTTGGCCGGATTATTCGT	TGCGCTCTTAGTTCAGTTCG	45.0	50.0	57.6	58.3	247-256 bp	1.0	81.5