Hotspot

trnF-GAA-ndhJ

LSC · 51256-51838 · 583 bp

Back to Aspidopterys

Pi 0.006

MarkerSeek score 63.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 7
pi: 0.006
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCGGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	694-739 bp	1.0	81.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CGGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	693-738 bp	1.0	81.6
trnF-GAA-ndhJ_p3	GTCGGAAATGGTCGGGATAG	CCGGAAAGTTGGATAGGGTG	55.0	55.0	57.8	58.0	743-788 bp	1.0	80.7
trnF-GAA-ndhJ_p4	GTCGGAAATGGTCGGGATAG	CGGAAAGTTGGATAGGGTGG	55.0	55.0	57.8	58.0	742-787 bp	1.0	80.7
trnF-GAA-ndhJ_p5	GGAAATGGTCGGGATAGCTC	CGGAAAGTTGGATAGGGTGG	55.0	55.0	57.8	58.0	739-784 bp	1.0	80.5