Hotspot

psbM-trnD-GUC

LSC · 31644-32816 · 1173 bp

Back to Aspidopterys

Pi 0.0119

MarkerSeek score 61.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1173
variable_sites: 28
pi: 0.0119
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGGACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.7	58.5	1263-1291 bp	1.0	68.9
psbM-trnD-GUC_p2	AGGGACTAGAATGAACAGTGC	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.4	58.5	1264-1292 bp	1.0	66.7
psbM-trnD-GUC_p3	AGGCAGTAGGGACTAGAATGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.3	58.5	1271-1299 bp	1.0	66.1
psbM-trnD-GUC_p4	GGGACTAGAATGAACAGTGCA	GTTCAATTGGTCAGAGCACC	47.6	50.0	57.7	57.0	1264-1292 bp	1.0	64.8
psbM-trnD-GUC_p5	AGGCAGTAGGGACTAGAATGAA	TTCAATTGGTCAGAGCACCG	45.5	50.0	58.0	58.5	1271-1299 bp	1.0	63.6