Hotspot

atpH-atpI

LSC · 14757-15735 · 979 bp

Back to Aspidopterys

Pi 0.00783

MarkerSeek score 65.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 979
variable_sites: 15
pi: 0.00783
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1066-1077 bp	1.0	80.3
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1058-1069 bp	1.0	80.1
atpH-atpI_p3	GTCCAATAGAAGCAAGCCCA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1104-1115 bp	1.0	79.5
atpH-atpI_p4	AAGCGGCAGAAATCAATGGA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1059-1070 bp	1.0	79.3
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1067-1078 bp	1.0	79.1