Hotspot

atpA-atpF

LSC · 12693-12751 · 59 bp

Back to Aspidopterys

Pi 0.0316

MarkerSeek score 78.00

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 3
pi: 0.0316
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	TACTAATCTCGTCGGCTCGA	TGTTTGGGTCGATGCAAGAA	50.0	45.0	57.8	58.0	119-122 bp	1.0	82.6
atpA-atpF_p2	CCGTCGCCTACTTGAAGTAC	TGTTTGGGTCGATGCAAGAA	55.0	45.0	58.1	58.0	201-204 bp	1.0	81.8
atpA-atpF_p3	GTCGCCTACTTGAAGTACGG	TGTTTGGGTCGATGCAAGAA	55.0	45.0	58.1	58.0	199-202 bp	1.0	81.8
atpA-atpF_p4	GTCGCCTACTTGAAGTACGG	AGTTGTTTGACCAGCGAGTT	55.0	45.0	58.1	58.0	251-254 bp	1.0	81.2
atpA-atpF_p5	CCGTCGCCTACTTGAAGTAC	AGTTGTTTGACCAGCGAGTT	55.0	45.0	58.1	58.0	253-256 bp	1.0	81.1