Hotspot

trnS-GCU-trnS-CGA

LSC · 8934-9845 · 912 bp

Back to Aspidopterys

Pi 0.0175
MarkerSeek score 62.80
Primer available yes
Label trnS-GCU-trnS-CGA

Feature Metadata

label_name
trnS-GCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
912
variable_sites
28
pi
0.0175
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnS-CGA_p1 CAATCCGACGCTTTAGTCCA CGAACGAATCACACTTTTACCA 50.0 40.9 58.0 57.3 956-1002 bp 1.0 63.5
trnS-GCU-trnS-CGA_p2 AATCCGACGCTTTAGTCCAC CGAACGAATCACACTTTTACCA 50.0 40.9 58.0 57.3 955-1001 bp 1.0 63.5
trnS-GCU-trnS-CGA_p3 GATCAACGGAACCGGAAAGA CGAACGAATCACACTTTTACCA 50.0 40.9 57.9 57.3 1021-1067 bp 1.0 62.5
trnS-GCU-trnS-CGA_p4 ATCAACGGAACCGGAAAGAG CGAACGAATCACACTTTTACCA 50.0 40.9 57.9 57.3 1020-1066 bp 1.0 62.4
trnS-GCU-trnS-CGA_p5 TGATCAACGGAACCGGAAAG CGAACGAATCACACTTTTACCA 50.0 40.9 58.2 57.3 1022-1068 bp 1.0 61.6