Hotspot

rps16-trnQ-UUG

LSC · 6431-7541 · 1111 bp

Back to Aspidopterys

Pi 0.0087

MarkerSeek score 62.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1111
variable_sites: 18
pi: 0.0087
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CAGCCGATCATGTCCTTCAA	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.0	58.0	1260-1280 bp	1.0	81.2
rps16-trnQ-UUG_p2	GCCGATCATGTCCTTCAAGT	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.0	58.0	1258-1278 bp	1.0	81.2
rps16-trnQ-UUG_p3	AATCCACAGCCGATCATGTC	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.0	58.0	1266-1286 bp	1.0	81.1
rps16-trnQ-UUG_p4	GAATCCACAGCCGATCATGT	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.0	58.0	1267-1287 bp	1.0	81.1
rps16-trnQ-UUG_p5	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1202-1222 bp	1.0	80.7