Hotspot

rps3

LSC · 84092-84754 · 663 bp

Back to Aspidistra

Pi 0.00409

MarkerSeek score 47.80

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 663
variable_sites: 21
pi: 0.00409
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TGTTCTATCACTGGACAGAATGG	AGACCCACTTGTCATATAACA	43.5	38.1	57.9	54.0	824-827 bp	1.0	43.6
rps3_p2	TGTTCTATCACTGGACAGAATGG	AAGACCCACTTGTCATATAACA	43.5	36.4	57.9	54.8	825-828 bp	1.0	43.6
rps3_p3	TGTTCTATCACTGGACAGAATGG	AGACCCACTTGTCATATAACAA	43.5	36.4	57.9	54.8	824-827 bp	1.0	43.6
rps3_p4	TGTTCTATCACTGGACAGAATGG	GACCCACTTGTCATATAACAATTGT	43.5	36.0	57.9	57.6	823-826 bp	1.0	43.6
rps3_p5	TGTTCTATCACTGGACAGAATGG	ACCCACTTGTCATATAACAATTGT	43.5	33.3	57.9	56.6	822-825 bp	1.0	43.6