Hotspot

rps15

SSC · 124900-125169 · 270 bp

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Pi 0.00381
MarkerSeek score 33.10
Primer available yes
Label rps15

Feature Metadata

label_name
rps15
feature_type
gene
region
SSC
length_bp
270
variable_sites
9
pi
0.00381
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15_p1 AAAAATGCAAACCAACCTTAA CCTGATCGGTAAAATCCACAGA 28.6 45.5 52.9 57.9 338-345 bp 1.0 44.2
rps15_p2 AAAATGCAAACCAACCTTAAA CCTGATCGGTAAAATCCACAGA 28.6 45.5 52.9 57.9 337-344 bp 1.0 44.2
rps15_p3 AAAATGCAAACCAACCTTAAA TCCTGATCGGTAAAATCCACAG 28.6 45.5 52.9 57.9 338-345 bp 1.0 44.2
rps15_p4 AAATGCAAACCAACCTTAAAA CCTGATCGGTAAAATCCACAGA 28.6 45.5 52.9 57.9 336-343 bp 1.0 44.2
rps15_p5 AAATGCAAACCAACCTTAAAA TCCTGATCGGTAAAATCCACAG 28.6 45.5 52.9 57.9 337-344 bp 1.0 44.2