Hotspot

infA

LSC · 81059-81291 · 233 bp

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Pi 0.00844

MarkerSeek score 42.50

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 233
variable_sites: 13
pi: 0.00844
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	AGCTTCGAGTTGTATCTCTCAC	TGGTGATCTTCCTCCGGTAT	45.5	50.0	57.6	57.5	393-401 bp	1.0	62.1
infA_p2	GCTTCGAGTTGTATCTCTCACC	TGGTGATCTTCCTCCGGTAT	50.0	50.0	58.6	57.5	392-400 bp	1.0	60.6
infA_p3	TCGAGTTGTATCTCTCACCA	TGGTGATCTTCCTCCGGTAT	45.0	50.0	55.3	57.5	389-397 bp	1.0	59.8
infA_p4	CTTCGAGTTGTATCTCTCACCA	TGGTGATCTTCCTCCGGTAT	45.5	50.0	57.3	57.5	391-399 bp	1.0	59.4
infA_p5	AGCTTCGAGTTGTATCTCTCA	TGGTGATCTTCCTCCGGTAT	42.9	50.0	56.2	57.5	393-401 bp	1.0	58.8