Hotspot

trnV-GAC-rps12

IRa · 139733-141612 · 1880 bp

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Pi 0.00156

MarkerSeek score 57.80

Primer available yes

Label trnV-GAC-rps12

Feature Metadata

label_name: trnV-GAC-rps12
feature_type: igs
region: IRa
length_bp: 1880
variable_sites: 18
pi: 0.00156
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps12_p1	GATGACTTCCACCACGTCAA	TGTGGCCAATACCAGGGATA	50.0	50.0	57.8	58.1	1950-2034 bp	1.0	82.6
trnV-GAC-rps12_p2	AACTGATGACTTCCACCACG	TGTGGCCAATACCAGGGATA	50.0	50.0	57.8	58.1	1954-2038 bp	1.0	82.6
trnV-GAC-rps12_p3	ATGACTTCCACCACGTCAAG	TGTGGCCAATACCAGGGATA	50.0	50.0	57.8	58.1	1949-2033 bp	1.0	82.6
trnV-GAC-rps12_p4	GATGACTTCCACCACGTCAA	AATTGTGGCCAATACCAGGG	50.0	50.0	57.8	58.1	1953-2037 bp	1.0	82.3
trnV-GAC-rps12_p5	ATGACTTCCACCACGTCAAG	AATTGTGGCCAATACCAGGG	50.0	50.0	57.8	58.1	1952-2036 bp	1.0	82.3