Hotspot

ccsA-ndhD

SSC · 116881-117111 · 231 bp

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Pi 0.0267

MarkerSeek score 68.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 231
variable_sites: 25
pi: 0.0267
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGAATTCGTCACTTGTAGCG	TCGCTATCAGTTGACAAGGT	50.0	45.0	57.3	56.6	403-404 bp	1.0	68.6
ccsA-ndhD_p2	CGAATTCGTCACTTGTAGCG	TTCGCTATCAGTTGACAAGGT	50.0	42.9	57.3	57.3	404-405 bp	1.0	66.5
ccsA-ndhD_p3	ATTCGTCACTTGTAGCGTCT	TCGCTATCAGTTGACAAGGT	45.0	45.0	57.0	56.6	400-401 bp	1.0	65.9
ccsA-ndhD_p4	CGAATTCGTCACTTGTAGCG	TCAGTTGACAAGGTACAAGCT	50.0	42.9	57.3	57.2	397-398 bp	1.0	65.3
ccsA-ndhD_p5	CGAATTCGTCACTTGTAGCG	TTTCGCTATCAGTTGACAAGGT	50.0	40.9	57.3	58.0	405-406 bp	1.0	63.9