Hotspot

rpl32-trnL-UAG

SSC · 114823-115750 · 928 bp

Back to Aspidistra

Pi 0.00288

MarkerSeek score 57.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 928
variable_sites: 27
pi: 0.00288
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTGCGACAAACAAATAAGAA	AGCGTGTCTACCGATTTCAC	33.3	50.0	54.6	58.0	985-993 bp	1.0	48.3
rpl32-trnL-UAG_p2	TGTGCGACAAACAAATAAGA	AGCGTGTCTACCGATTTCAC	35.0	50.0	53.8	58.0	985-993 bp	0.96875	48.0
rpl32-trnL-UAG_p3	TGTGCGACAAACAAATAAGA	CAGCGTGTCTACCGATTTCA	35.0	50.0	53.8	58.0	986-994 bp	0.96875	48.0
rpl32-trnL-UAG_p4	TGTGCGACAAACAAATAAGA	TTTACAGTATGCCGCCACTC	35.0	50.0	53.8	58.0	1045-1053 bp	0.96875	47.8
rpl32-trnL-UAG_p5	TGTGCGACAAACAAATAAGA	TCCTAAGAGCAGCGTGTCTA	35.0	50.0	53.8	57.9	995-1003 bp	0.96875	47.1