Hotspot

rpl20-rps12

LSC · 70205-70947 · 743 bp

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Pi 0.00695

MarkerSeek score 58.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 29
pi: 0.00695
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ATGCGCCCCTATAAAGGTTG	TCGGGGAACATGTACTAGGG	50.0	55.0	58.0	58.2	852-857 bp	0.96875	80.1
rpl20-rps12_p2	TTTTTGTTCGACGTCTCCGA	TCGGGGAACATGTACTAGGG	45.0	55.0	57.8	58.2	817-822 bp	1.0	79.7
rpl20-rps12_p3	TTTTGTTCGACGTCTCCGAG	TCGGGGAACATGTACTAGGG	50.0	55.0	58.2	58.2	816-821 bp	1.0	79.4
rpl20-rps12_p4	AGTAAGTCTTGAATGCGCCC	TCGGGGAACATGTACTAGGG	50.0	55.0	58.3	58.2	864-869 bp	0.96875	78.2
rpl20-rps12_p5	TTCGACGTCTCCGAGCTATA	TCGGGGAACATGTACTAGGG	50.0	55.0	57.8	58.2	811-816 bp	0.96875	77.9