Hotspot

psaJ-rpl33

LSC · 68340-68866 · 527 bp

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Pi 0.00439

MarkerSeek score 57.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 527
variable_sites: 20
pi: 0.00439
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATATCTTTCCACAGCGCCTG	AGAAATGCCTGGCGATTCTT	50.0	45.0	58.0	57.9	706-754 bp	1.0	85.5
psaJ-rpl33_p2	TTGGGTCTTTAGCGGGTCTA	AGAAATGCCTGGCGATTCTT	50.0	45.0	58.1	57.9	668-716 bp	1.0	85.5
psaJ-rpl33_p3	CATATCTTTCCACAGCGCCT	AGAAATGCCTGGCGATTCTT	50.0	45.0	58.0	57.9	707-755 bp	1.0	85.4
psaJ-rpl33_p4	ATGGTTTGGGTCTTTAGCGG	AGAAATGCCTGGCGATTCTT	50.0	45.0	58.2	57.9	673-721 bp	1.0	84.6
psaJ-rpl33_p5	TGGGTCTTTAGCGGGTCTAT	AGAAATGCCTGGCGATTCTT	50.0	45.0	57.8	57.9	667-715 bp	1.0	84.6