Hotspot

petA-psbJ

LSC · 63878-64969 · 1092 bp

Back to Aspidistra

Pi 0.00715

MarkerSeek score 68.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1092
variable_sites: 48
pi: 0.00715
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCCCAAGGCCTTTTGTTTTT	CCGGATTGGGTTCATCTCTG	45.0	55.0	57.9	58.0	1110-1228 bp	1.0	86.5
petA-psbJ_p2	GGCATCCGTTATTTTGGCAC	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.1	58.0	1087-1205 bp	1.0	86.3
petA-psbJ_p3	GCCCAAGGCCTTTTGTTTTT	TCCGGATTGGGTTCATCTCT	45.0	50.0	57.9	58.1	1111-1229 bp	1.0	85.8
petA-psbJ_p4	GGCATCCGTTATTTTGGCAC	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.1	58.1	1088-1206 bp	1.0	85.7
petA-psbJ_p5	TGGCATCCGTTATTTTGGCA	CCGGATTGGGTTCATCTCTG	45.0	55.0	58.4	58.0	1088-1206 bp	1.0	83.4