Hotspot

ycf4-cemA

LSC · 61129-61996 · 868 bp

Back to Aspidistra

Pi 0.00421

MarkerSeek score 64.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 868
variable_sites: 37
pi: 0.00421
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.9	57.8	954-987 bp	1.0	85.5
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	ATGAGAGAGAGACCCACCAG	50.0	55.0	58.1	57.8	956-989 bp	1.0	85.2
ycf4-cemA_p3	AAAAAGCTGCTGAATTGGCC	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.8	57.8	981-1014 bp	1.0	84.8
ycf4-cemA_p4	TGACTCCGCGAGAAATTGAG	ATGAGAGAGAGACCCACCAG	50.0	55.0	58.0	57.8	1002-1035 bp	0.96875	84.7
ycf4-cemA_p5	TGAGAATTTGACTCCGCGAG	ATGAGAGAGAGACCCACCAG	50.0	55.0	58.0	57.8	1010-1043 bp	0.96875	84.7