Hotspot

atpB-rbcL

LSC · 55322-56106 · 785 bp

Back to Aspidistra

Pi 0.00271

MarkerSeek score 56.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 785
variable_sites: 22
pi: 0.00271
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACA	45.0	45.0	57.9	56.6	881-908 bp	1.0	72.7
atpB-rbcL_p2	CTGGGCCAATAATTTGAGCG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	57.5	56.6	882-909 bp	1.0	69.8
atpB-rbcL_p3	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACAT	45.0	42.9	57.9	57.2	881-908 bp	1.0	68.9
atpB-rbcL_p4	CGGGGGAAAAACAACATCCA	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	905-932 bp	1.0	68.3
atpB-rbcL_p5	TTCGTCAAGTGTGGAAACCG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	845-872 bp	0.96875	67.0