Pi
0.00609
MarkerSeek score
68.20
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1038-1129 bp | 1.0 | 85.6 |
| trnT-UGU-trnL-UAA_p2 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 1046-1137 bp | 1.0 | 85.1 |
| trnT-UGU-trnL-UAA_p3 | AAATGCGATGCTCTAACCCC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.3 | 58.0 | 1010-1101 bp | 1.0 | 85.0 |
| trnT-UGU-trnL-UAA_p4 | AAATGCGATGCTCTAACCCC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.3 | 58.1 | 1018-1109 bp | 1.0 | 84.5 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | ATCCAATCAAGTCCGTAGCG | 50.0 | 50.0 | 57.8 | 57.8 | 1061-1152 bp | 1.0 | 83.8 |