Hotspot

trnC-GCA-petN

LSC · 28319-29238 · 920 bp

Back to Aspidistra

Pi 0.00656

MarkerSeek score 53.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 920
variable_sites: 48
pi: 0.00656
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTA	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.5	58.0	998-1003 bp	1.0	80.1
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTA	AGCAATTAGTAGTGCCCAGC	50.0	50.0	57.5	58.0	1075-1080 bp	1.0	79.6
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	AGTCCACTCCTTCCCCATAC	50.0	55.0	57.5	58.1	1050-1055 bp	1.0	79.1
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTA	GTCCACTCCTTCCCCATACT	50.0	55.0	57.5	58.1	1049-1054 bp	1.0	79.1
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTA	GAGTCCACTCCTTCCCCATA	50.0	55.0	57.5	57.8	1051-1056 bp	1.0	78.6