Hotspot

atpF-atpH

LSC · 13525-14035 · 511 bp

Back to Aspidistra

Pi 0.00473

MarkerSeek score 59.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 511
variable_sites: 23
pi: 0.00473
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	AATTTACGGACTGGTCGTGG	50.0	50.0	57.9	57.9	613-635 bp	1.0	85.2
atpF-atpH_p2	CAGTGGCCTAAGGAAACGAA	AATTTACGGACTGGTCGTGG	50.0	50.0	57.8	57.9	585-607 bp	1.0	85.0
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.9	58.3	599-621 bp	1.0	83.7
atpF-atpH_p4	CAGTGGCCTAAGGAAACGAA	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.8	58.3	571-593 bp	1.0	83.6
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CGTGGCATTAGCGCTTTTAT	50.0	45.0	57.9	57.2	598-620 bp	1.0	79.9