Hotspot

rps16-trnQ-UUG

LSC · 5810-6797 · 988 bp

Back to Aspidistra

Pi 0.00558

MarkerSeek score 61.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 988
variable_sites: 37
pi: 0.00558
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1059-1075 bp	1.0	82.1
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1070-1086 bp	1.0	81.5
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1063-1079 bp	1.0	77.4
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1089-1105 bp	1.0	77.2
rps16-trnQ-UUG_p5	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.2	58.1	1071-1087 bp	1.0	77.0