Hotspot

matK-trnK-UUU

LSC · 3228-4001 · 774 bp

Back to Aspidistra

Pi 0.00427

MarkerSeek score 59.70

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 27
pi: 0.00427
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TAGGAAGTGTTGTTGCCGAG	TCTGGGTTGCTAACTCAACG	50.0	50.0	57.8	57.8	865-877 bp	1.0	81.3
matK-trnK-UUU_p2	TAGGAAGTGTTGTTGCCGAG	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.8	58.2	849-861 bp	1.0	81.3
matK-trnK-UUU_p3	TAGGAAGTGTTGTTGCCGAG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.8	58.2	848-860 bp	1.0	81.3
matK-trnK-UUU_p4	TAGGAAGTGTTGTTGCCGAG	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.8	57.5	862-874 bp	1.0	78.9
matK-trnK-UUU_p5	GGAAGTGTTGTTGCCGAGAT	TCTGGGTTGCTAACTCAACG	50.0	50.0	58.5	57.8	863-875 bp	1.0	78.8