Hotspot

ycf1

SSC · 125536-130959 · 5424 bp

Back to Asparagus

Pi 0.00844

MarkerSeek score 53.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5424
variable_sites: 341
pi: 0.00844
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTTTGATGTTCCAATTCTTGAGA	AGAAAAATACTTACCCAAAAGG	32.0	31.8	57.2	52.3	191 bp	1.0	48.0
ycf1_p2	TGATGTTCCAATTCTTGAGA	AGAAAAATACTTACCCAAAAGG	35.0	31.8	52.1	52.3	186 bp	1.0	48.0
ycf1_p3	TCTTTTGATGTTCCAATTCTTGAGA	AAGAAAAATACTTACCCAAAAGG	32.0	30.4	57.2	53.1	192 bp	1.0	48.0
ycf1_p4	TGATGTTCCAATTCTTGAGAA	AGAAAAATACTTACCCAAAAGG	33.3	31.8	53.0	52.3	186 bp	1.0	48.0
ycf1_p5	TTGATGTTCCAATTCTTGAGA	AGAAAAATACTTACCCAAAAGG	33.3	31.8	53.0	52.3	187 bp	1.0	48.0