Hotspot

ndhD

SSC · 117121-118626 · 1506 bp

Back to Asparagus

Pi 0.00462

MarkerSeek score 46.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 52
pi: 0.00462
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACGAAAACTTTGATTTCTGTCTCA	TTACCGACAAAAGCCCGTAC	33.3	50.0	57.3	58.2	1617-1632 bp	1.0	44.6
ndhD_p2	ACGAAAACTTTGATTTCTGTCTCA	TTTTACCGACAAAAGCCCGT	33.3	45.0	57.3	58.3	1619-1634 bp	1.0	43.7
ndhD_p3	ACGAAAACTTTGATTTCTGTCTCA	AAAGCCCGTACTCGCTAAAA	33.3	45.0	57.3	57.5	1608-1623 bp	1.0	43.7
ndhD_p4	ACGAAAACTTTGATTTCTGTCTCA	CAAAAGCCCGTACTCGCTAA	33.3	50.0	57.3	58.3	1625 bp	0.028571	1.5
ndhD_p5	ACGAAAACTTTGATTTCTGTCTCA	AAAAGCCCGTACTCGCTAAA	33.3	45.0	57.3	57.5	1624 bp	0.028571	1.1