Hotspot

rpl32-trnL-UAG

SSC · 114958-115765 · 808 bp

Back to Asparagus

Pi 0.0126
MarkerSeek score 67.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
808
variable_sites
102
pi
0.0126
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CAAAGTCAGTTTCCACCGGA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 907-982 bp 1.0 91.6
rpl32-trnL-UAG_p2 CAAAGTCAGTTTCCACCGGA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 908-983 bp 1.0 91.6
rpl32-trnL-UAG_p3 CAAAGTCAGTTTCCACCGGA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 917-992 bp 1.0 90.7
rpl32-trnL-UAG_p4 CAAAGTCAGTTTCCACCGGA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.0 57.8 916-991 bp 1.0 90.1
rpl32-trnL-UAG_p5 CAAAGTCAGTTTCCACCGGA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 915-990 bp 1.0 90.1