Hotspot

ndhF-rpl32

SSC · 113759-114783 · 1025 bp

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Pi 0.0116
MarkerSeek score 62.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1025
variable_sites
81
pi
0.0116
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCTGTCGGAATAAGCA TGCAGCTAAAGATCCCTTTTTC 45.0 40.9 57.8 56.9 1160-1208 bp 1.0 63.9
ndhF-rpl32_p2 TGTTGCTGTCGGAATAAGCA TGCAGCTAAAGATCCCTTTT 45.0 40.0 57.8 54.9 1160-1208 bp 1.0 63.6
ndhF-rpl32_p3 TGTTGCTGTCGGAATAAGCA TTGCAGCTAAAGATCCCTTT 45.0 40.0 57.8 54.9 1161-1209 bp 1.0 63.6
ndhF-rpl32_p4 TGTTGCTGTCGGAATAAGCA TGCAGCTAAAGATCCCTTTTT 45.0 38.1 57.8 55.7 1160-1208 bp 1.0 62.2
ndhF-rpl32_p5 TGTTGCTGTCGGAATAAGCA TTGCAGCTAAAGATCCCTTTT 45.0 38.1 57.8 55.7 1161-1209 bp 1.0 62.2