Hotspot

ycf4-cemA

LSC · 61436-61983 · 548 bp

Back to Asparagus

Pi 0.00498

MarkerSeek score 58.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 548
variable_sites: 28
pi: 0.00498
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	AAGGAAAGAGAGACCGACCA	45.0	50.0	57.9	58.0	559-660 bp	0.971429	86.1
ycf4-cemA_p2	TCCGCGGGAAATTGAGAAAA	AAGGAAAGAGAGACCGACCA	45.0	50.0	58.1	58.0	603-704 bp	0.971429	85.8
ycf4-cemA_p3	CTTGCGCGTACCAATTGAAG	AAGGAAAGAGAGACCGACCA	50.0	50.0	58.1	58.0	561-662 bp	0.971429	85.8
ycf4-cemA_p4	AATTTGACTCCGCGGGAAAT	AAGGAAAGAGAGACCGACCA	45.0	50.0	58.2	58.0	611-712 bp	0.971429	85.2
ycf4-cemA_p5	ATTTGACTCCGCGGGAAATT	AAGGAAAGAGAGACCGACCA	45.0	50.0	58.2	58.0	610-711 bp	0.971429	85.2