Hotspot

trnT-GGU-psbD

LSC · 32281-33326 · 1046 bp

Back to Asparagus

Pi 0.0048

MarkerSeek score 59.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1046
variable_sites: 38
pi: 0.0048
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCACCCTACAAAAACGA	55.0	50.0	58.1	57.7	1186-1236 bp	1.0	84.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCACCCTACAAAAACGA	50.0	50.0	58.1	57.7	1189-1239 bp	0.971429	82.5
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GGACCACCCTACAAAAACGA	50.0	50.0	58.3	57.7	1163-1213 bp	1.0	82.2
trnT-GGU-psbD_p4	GGCCCTTTTAACTCAGTGGT	GGACCACCCTACAAAAACGA	50.0	50.0	57.7	57.7	1201-1251 bp	1.0	82.2
trnT-GGU-psbD_p5	TGGTAGAGTAACGCCATGGT	GGACCACCCTACAAAAACGA	50.0	50.0	58.4	57.7	1185-1235 bp	1.0	81.0