Hotspot

psbM-trnD-GUC

LSC · 30043-31112 · 1070 bp

Back to Asparagus

Pi 0.00675

MarkerSeek score 60.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1070
variable_sites: 56
pi: 0.00675
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.3	58.5	1157-1196 bp	1.0	80.4
psbM-trnD-GUC_p2	TGAACAGCGCAGTAGCAATA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	1156-1195 bp	1.0	79.4
psbM-trnD-GUC_p3	ACAGCGCAGTAGCAATAAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	1153-1192 bp	1.0	79.4
psbM-trnD-GUC_p4	ATGAACAGCGCAGTAGCAAT	TGCTATACACCTCACTGGGA	45.0	50.0	58.3	57.5	1183-1222 bp	0.914286	76.2
psbM-trnD-GUC_p5	TGAACAGCGCAGTAGCAATA	TGCTATACACCTCACTGGGA	45.0	50.0	57.6	57.5	1182-1221 bp	0.914286	75.2