Hotspot

atpH-atpI

LSC · 14014-14510 · 497 bp

Back to Asparagus

Pi 0.00652

MarkerSeek score 60.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 497
variable_sites: 26
pi: 0.00652
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TAGGGGAATCAATGGAGGGT	45.0	50.0	58.0	57.4	546-565 bp	1.0	81.2
atpH-atpI_p2	CGCAATACCTTCTACGGCTT	TAGGGGAATCAATGGAGGGT	50.0	50.0	58.1	57.4	634-653 bp	1.0	80.3
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	573-592 bp	0.971429	80.3
atpH-atpI_p4	CGCAATACCTTCTACGGCTT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.1	58.5	661-680 bp	0.971429	79.5
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	629-648 bp	0.971429	79.2