Hotspot

trnS-GCU-trnG-UCC

LSC · 8300-9179 · 880 bp

Back to Asparagus

Pi 0.0146

MarkerSeek score 75.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 81
pi: 0.0146
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	AATCAGAGGAAGCGGAGAGA	ACGAATCGCACTTTTACCACT	50.0	42.9	57.8	58.2	999-1119 bp	1.0	79.2
trnS-GCU-trnG-UCC_p2	AATCAGAGGAAGCGGAGAGA	ACGAATCGCACTTTTACCAC	50.0	45.0	57.8	56.8	999-1119 bp	1.0	79.2
trnS-GCU-trnG-UCC_p3	AATCTGCCGCTTTAGTCCAC	ACGAATCGCACTTTTACCACT	50.0	42.9	58.3	58.2	933-1053 bp	1.0	78.7
trnS-GCU-trnG-UCC_p4	CAATCTGCCGCTTTAGTCCA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.3	58.2	934-1054 bp	1.0	78.7
trnS-GCU-trnG-UCC_p5	AATCTGCCGCTTTAGTCCAC	ACGAATCGCACTTTTACCAC	50.0	45.0	58.3	56.8	933-1053 bp	1.0	78.6