Hotspot

ycf4

LSC · 67439-67990 · 552 bp

Back to Asclepias

Pi 0.0697

MarkerSeek score 77.90

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 552
variable_sites: 38
pi: 0.0697
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	TCTCGATTCCAATAAAATAAAATCGGA	GCATTTTTGCAAACCCCCAT	29.6	45.0	57.7	58.1	631-641 bp	1.0	45.8
ycf4_p2	CTCGATTCCAATAAAATAAAATCGGA	GCATTTTTGCAAACCCCCAT	30.8	45.0	56.5	58.1	630-640 bp	1.0	45.8
ycf4_p3	TCTCGATTCCAATAAAATAAAATCGG	GCATTTTTGCAAACCCCCAT	30.8	45.0	56.5	58.1	631-641 bp	1.0	45.8
ycf4_p4	TCGATTCCAATAAAATAAAATCGGA	GCATTTTTGCAAACCCCCAT	28.0	45.0	55.5	58.1	629-639 bp	1.0	45.8
ycf4_p5	GTTTCTCTCGATTCCAATAAAA	GCATTTTTGCAAACCCCCAT	31.8	45.0	52.5	58.1	636-646 bp	1.0	45.7