Hotspot

ndhD

SSC · 123399-124901 · 1503 bp

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Pi 0.00599

MarkerSeek score 49.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 9
pi: 0.00599
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CTAACGAAGCTTGGGGATCC	CAAATTGCTTCGGCTCCAAG	55.0	50.0	58.0	58.0	2260-2263 bp	1.0	80.7
ndhD_p2	CTAACGAAGCTTGGGGATCC	AAGATGTGAATCCGCCTGTC	55.0	50.0	58.0	58.0	2216-2219 bp	1.0	80.6
ndhD_p3	CTAACGAAGCTTGGGGATCC	AACAGAAGACTGCGTTGGTT	55.0	45.0	58.0	58.0	2240-2243 bp	1.0	80.5
ndhD_p4	TTCGGCAATTGTAGCGTGTA	CAAATTGCTTCGGCTCCAAG	45.0	50.0	57.9	58.0	2139-2142 bp	1.0	80.5
ndhD_p5	TTCGGCAATTGTAGCGTGTA	AAGATGTGAATCCGCCTGTC	45.0	50.0	57.9	58.0	2095-2098 bp	1.0	80.4