Hotspot

accD

LSC · 64275-66593 · 2319 bp

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Pi 0.0168
MarkerSeek score 55.00
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
2319
variable_sites
39
pi
0.0168
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 AAATAGGGGGCAAGAGAACT ACTAACTACTCCTTCGCTACA 45.0 42.9 56.1 55.6 2373-2658 bp 1.0 41.2
accD_p2 AATAGGGGGCAAGAGAACTT ACTAACTACTCCTTCGCTACA 45.0 42.9 56.1 55.6 2372-2657 bp 1.0 41.2
accD_p3 ATAGGGGGCAAGAGAACTTT ACTAACTACTCCTTCGCTACA 45.0 42.9 56.1 55.6 2371-2656 bp 1.0 41.2
accD_p4 AAATAGGGGGCAAGAGAACTT ACTAACTACTCCTTCGCTACA 42.9 42.9 56.9 55.6 2373-2658 bp 1.0 41.2
accD_p5 AATAGGGGGCAAGAGAACTTT ACTAACTACTCCTTCGCTACA 42.9 42.9 56.9 55.6 2372-2657 bp 1.0 41.2