Hotspot

ccsA-ndhD

SSC · 123148-123398 · 251 bp

Back to Asclepias

Pi 0.0398

MarkerSeek score 61.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 251
variable_sites: 10
pi: 0.0398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CTAACGAAGCTTGGGGATCC	ATGAGTGGCTTTGTTGCTGA	55.0	45.0	58.0	58.0	820-824 bp	1.0	81.0
ccsA-ndhD_p2	TTCGGCAATTGTAGCGTGTA	ATGAGTGGCTTTGTTGCTGA	45.0	45.0	57.9	58.0	699-703 bp	1.0	80.8
ccsA-ndhD_p3	CTAACGAAGCTTGGGGATCC	TATCTTGACGAAATGGGCGG	55.0	50.0	58.0	58.1	913-917 bp	1.0	80.5
ccsA-ndhD_p4	CTAACGAAGCTTGGGGATCC	TGACGAAATGGGCGGAATAG	55.0	50.0	58.0	58.1	908-912 bp	1.0	80.5
ccsA-ndhD_p5	TTTTTACCCAGGGCTTTGCT	ATGAGTGGCTTTGTTGCTGA	45.0	45.0	57.9	58.0	1231-1235 bp	1.0	80.5