Hotspot

rpl32-trnL-UAG

SSC · 121077-122005 · 929 bp

Back to Asclepias

Pi 0.0227

MarkerSeek score 67.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 929
variable_sites: 20
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGAAGGGATATTGGACAGCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.0	57.9	1036-1063 bp	1.0	76.1
rpl32-trnL-UAG_p2	GGAAGGGATATTGGACAGCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.0	57.8	1029-1056 bp	1.0	75.7
rpl32-trnL-UAG_p3	GGAAGGGATATTGGACAGCA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.0	57.8	1035-1062 bp	1.0	75.5
rpl32-trnL-UAG_p4	GGAAGGGATATTGGACAGCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.0	57.8	1034-1061 bp	1.0	75.5
rpl32-trnL-UAG_p5	GGAAGGGATATTGGACAGCA	GAGCAGCGTGTCTACCAATT	50.0	50.0	57.0	58.3	1030-1057 bp	1.0	74.9