Hotspot

ycf1-ndhF

SSC · 117347-117934 · 588 bp

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Pi 0.031

MarkerSeek score 72.30

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 588
variable_sites: 17
pi: 0.031
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TGGTCCTGAAAAAGGCTTCC	ATCAATGGCGTTGGGTTTCT	50.0	45.0	58.0	58.1	1084-1247 bp	1.0	84.7
ycf1-ndhF_p2	GGCCGTACGAACAGAAATCT	ATCAATGGCGTTGGGTTTCT	50.0	45.0	58.0	58.1	997-1160 bp	1.0	84.7
ycf1-ndhF_p3	GGCCGTACGAACAGAAATCT	ACCAGGGGCATTAGAGGATT	50.0	50.0	58.0	58.1	1063-1226 bp	1.0	84.6
ycf1-ndhF_p4	TGGTCCTGAAAAAGGCTTCC	AACCAGGGGCATTAGAGGAT	50.0	50.0	58.0	58.1	1151-1314 bp	1.0	84.5
ycf1-ndhF_p5	TGGTCCTGAAAAAGGCTTCC	ACCAGGGGCATTAGAGGATT	50.0	50.0	58.0	58.1	1150-1313 bp	1.0	84.5