Hotspot

rpl20-rps12

LSC · 76364-77278 · 915 bp

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Pi 0.0057

MarkerSeek score 64.40

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 915
variable_sites: 5
pi: 0.0057
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTAAGTCTTGAATGGGCCCC	CTCAACGTCGAGGAACATGT	55.0	50.0	58.2	57.9	1004-1042 bp	1.0	80.9
rpl20-rps12_p2	CCCCTCGAAAGCTTAATGCA	CTCAACGTCGAGGAACATGT	50.0	50.0	58.2	57.9	988-1026 bp	1.0	80.9
rpl20-rps12_p3	TAAGTCTTGAATGGGCCCCT	CTCAACGTCGAGGAACATGT	50.0	50.0	58.3	57.9	1003-1041 bp	1.0	80.3
rpl20-rps12_p4	GTAAGTCTTGAATGGGCCCC	GAGGAACATGTACTCGGGTG	55.0	55.0	58.2	57.7	995-1033 bp	1.0	79.1
rpl20-rps12_p5	CCCCTCGAAAGCTTAATGCA	GAGGAACATGTACTCGGGTG	50.0	55.0	58.2	57.7	979-1017 bp	1.0	79.1